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Oleic Acid

Synonyms: Oleate, 9-Octadecenoic Acid, Elaidoic acid

Oleic acid (Oleate, 9-Octadecenoic Acid, Elaidoic acid) is a fatty acid that occurs naturally in various animal and vegetable fats and oils. It is used commercially in the preparation of oleates and lotions, and as a pharmaceutical solvent. Oleic acid is an abundant monounsaturated fatty acid. Oleic acid is a Na+/K+ ATPase activator.

Oleic Acid Chemical Structure

Oleic Acid Chemical Structure

CAS No. 112-80-1

Purity & Quality Control

Oleic Acid Related Products

Signaling Pathway

ATPase Signaling Pathways

ATPase Signaling Pathway

Biological Activity

Description Oleic acid (Oleate, 9-Octadecenoic Acid, Elaidoic acid) is a fatty acid that occurs naturally in various animal and vegetable fats and oils. It is used commercially in the preparation of oleates and lotions, and as a pharmaceutical solvent. Oleic acid is an abundant monounsaturated fatty acid. Oleic acid is a Na+/K+ ATPase activator.
In vitro
In vitro Oleic acid treatment induces a marked increase in the proliferation of 786-O cells in a dose-dependent manner. It delays 786-O cell apoptosis. In 786‑O cells, ILK is upregulated by oleic acid treatment. Oleic acid activates G protein-coupled receptors and phosphorylates ERK1/2 to induce cancer cell proliferation in breast cancer[1]. Oleic acid(as low as 5 M) inhibits the stearic acid-induced increase in intercellular adhesion molecule-1 (ICAM-1) expression. Oleic acid is able to diminish endothelial cell activation, irrespective of the stimulus utilized, by suppressing the upregulation of adhesion molecules. It can protect Chinese hamster ovary cells from palmitate-induced lipotoxicity by promoting triglyceride synthesis within the cells[2].
Cell Research Cell lines Human RCC（renal cell carcinoma） cell line, 786-O.
Concentrations 0.05, 0.1, 0.2 mmol/l
Incubation Time 48 h
Method

Cells (2x103 cells/well) are seeded in 96-well microtitre plates and incubated for 24 h in 100 µl culture medium. Cells in the experimental group were then treated with 0.05, 0.1 or 0.2 mmol/l oleic acid for 48 h. MTT [20 µl (5 g/l)] is added to the cells which were then cultivated for a further 4 h. Following the removal of the supernatant fluid, 150 µl/well DMSO is added to the cells which are agitated for 15 min. Absorbance is measured at 490 nm by an ELISA reader.
In Vivo
In vivo Oleic acid inhibits in vivo Na/K-ATPase activity in the lungs and induced lung injury. 10 μM oleic acid induces lung edema and inflammation with cell influx, lipid body formation, and leukotriene B4 (LTB4) and prostaglandin E2 (PGE2) production[3]. Oleic acid ameliorates amyloidosis in mouse models of Alzheimer's disease[4].
Animal Research Animal Models male Swiss mice
Dosages 2.5, 5.0 or 10.0 μmol
Administration i.v.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06274788 Not yet recruiting
Parenteral Nutrition Associated Liver Disease (PNALD)|Essential Fatty Acid Deficiency|Malnutrition|Pediatric ALL
Fresenius Kabi
 May 1 2024 Phase 4
NCT05556967 Completed
Adherence Patient
University of Mississippi Medical Center|University of Mississippi National Center for Natural Products Research|National Center for Complementary and Integrative Health (NCCIH)
 August 9 2023 Not Applicable
NCT04614233 Active not recruiting
Overweight and Obesity
Yale University|National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
 April 6 2021 Phase 4
NCT04053569 Unknown status
Metabolic Syndrome Protection Against
Azienda Ospedaliera Specializzata in Gastroenterologia Saverio de Bellis
 October 1 2019 Not Applicable
NCT05791370 Completed
Diet|Healthy
Malaysia Palm Oil Board|Universiti Putra Malaysia
 January 1 2019 Not Applicable

Chemical Information & Solubility

Molecular Weight 282.46 Formula

C18H34O2
CAS No. 112-80-1 SDF Download Oleic Acid SDF
Density 0.89 g/mL at 25 °C
Smiles CCCCCCCCC=CCCCCCCCC(=O)O
Storage (From the date of receipt) 2 years -80°C liquid

In vitro
Batch:

Ethanol : 100 mg/mL

DMSO : 50 mg/mL ( (177.01 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


Molecular Weight Calculator

In vivo
Batch:

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In vivo Formulation Calculator

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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